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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
05/02/2013 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
SOUZA, I. I. F.; MELO, E. S. P.; RAMOS, C. A. M.; FARIAS, T. A.; OSORIO, A. L. A. R.; JORGE, K. S. G.; VIDAL, C. E. S.; SILVA, A. S.; SILVA, M. R.; PELLEGRIN, A. O.; ARAUJO, F. R. |
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Afiliação: |
INGRID I. F. SOUZA, UFMS; ELAINE S. P. MELO, CNPQ; CARLOS A. M. RAMOS, CNPQ; THAIS A. FARIAS, UFMS; ANA LUISA A. L. OSORIO, UFMS; KLAUDIA S. G. JORGE, UFMS; CARLOS E. S. VIDAL, UFSM; ALTINO A. SILVA, IFET/ES; MARCIO ROBERTO SILVA, CNPGL; AIESCA OLIVEIRA PELLEGRIN, CPAP; FLABIO RIBEIRO ARAUJO, CNPGC. |
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Título: |
Screening of recombinant proteins as antigens in indirect elisa for diagnosis of bovine tuberculosis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
SpringerPlus, v. 1, n. 1, article 77, 2012. |
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DOI: |
https://doi.org/10.1186%2F2193-1801-1-77 |
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Idioma: |
Inglês |
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Notas: |
Disponível em: http://www.springerplus.com/content/1/1/77 |
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Conteúdo: |
Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test. |
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Thesagro: |
Bovino; Elisa; Mycobacterium Bovis; Sanidade Animal; Tuberculose. |
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Thesaurus NAL: |
cattle; recombinant proteins; serology. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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008 2012 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186%2F2193-1801-1-77$2DOI
100 1 $aSOUZA, I. I. F.
245 $aScreening of recombinant proteins as antigens in indirect elisa for diagnosis of bovine tuberculosis.$h[electronic resource]
260 $c2012
500 $aDisponível em: http://www.springerplus.com/content/1/1/77
520 $aBovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test.
650 $acattle
650 $arecombinant proteins
650 $aserology
650 $aBovino
650 $aElisa
650 $aMycobacterium Bovis
650 $aSanidade Animal
650 $aTuberculose
700 1 $aMELO, E. S. P.
700 1 $aRAMOS, C. A. M.
700 1 $aFARIAS, T. A.
700 1 $aOSORIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aVIDAL, C. E. S.
700 1 $aSILVA, A. S.
700 1 $aSILVA, M. R.
700 1 $aPELLEGRIN, A. O.
700 1 $aARAUJO, F. R.
773 $tSpringerPlus$gv. 1, n. 1, article 77, 2012.
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